Appearance of solution
Dissolve, with heating at 50
, 10.0 g of Galactose in 50 mL of carbon dioxide-free water. The solution is not more intensely colored than a solution prepared immediately before use by mixing 3.0 mL of ferric chloride CS, 3.0 mL of cobaltous chloride CS, and 2.4 mL of cupric sulfate CS with dilute hydrochloric acid (10 g per L) to make 10 mL, and diluting 1.5 mL of this solution with the dilute hydrochloric acid to 100 mL. Make the comparison by viewing the substance and the solution downward in matched color-comparison tubes against a white surface (see Color and Achromicity 631
Specific rotation 781S:
Transfer 10.0 g to a 100-mL volumetric flask, and dissolve in 80 mL of water. Add 0.2 mL of ammonia TS, allow to stand for 30 minutes, then dilute with water to volume.
Microbial limits 61
It meets the requirements of the test for absence of Salmonella
species, Escherichia coli, Staphylcoccus aureus,
and Pseudomonas aeruginosa.
The total aerobic microbial count does not exceed 1000 cfu per g, and the total combined molds and yeasts count does not exceed 100 cfu per g.
Dissolve 10.0 g, with heating at 50
, in 40 mL of carbon dioxide-free water. Dilute with carbon dioxide-free water to 50 mL [NOTE
Use this solution for the Barium
. To 30 mL of this solution, add 0.3 mL of phenolphthalein TS, and titrate with 0.01 N sodium hydroxide to a pink color: not more than 1.5 mL of 0.01 N sodium hydroxide is required to produce a pink color.
Add 6 mL of water to 5 mL of the solution prepared for the Acidity test.
Add 5 mL of water and 1 mL of dilute sulfuric acid to 5 mL of the solution prepared for the Acidity test. Allow to stand for 1 hour: any opalescence in the Test solution is not more intense than that of the Standard solution.
Limit of lead
Dilute 12 mL of acetic acid with water to 100 mL. Mix equal parts of this solution and water to prepare the Diluent.
Lead standard stock solution
Transfer an accurately weighed quantity of lead nitrate, about 400 mg, to a 250-mL volumetric flask, dilute with water to volume, and mix.
Lead standard solution
Dilute 1.0 mL of Lead standard stock solution with water to 10 mL. Dilute 1.0 mL of this solution with water to 10 mL.
To three identical flasks, add 0.5 mL, 1.0 mL, and 1.5 mL of Lead standard solution, respectively, and then add to each flask 20.0 g of galactose. Dilute with Diluent to 100 mL. To each flask add 2.0 mL of ammonium pyrrolidinedithiocarbamate solution (10 g per L) and 10.0 mL of methyl isobutyl ketone, then shake for 30 seconds. [NOTEProtect from light.] Allow the layers to separate, and use the methyl isobutyl ketone (upper) layer for analysis.
Dissolve 20.0 g of Galactose in Diluent, and dilute with Diluent to 100 mL. Add 2.0 mL of ammonium pyrrolidinedithiocarbamate solution (10 g per L) and 10.0 mL of methyl isobutyl ketone, and shake for 30 seconds. [NOTEProtect from light.] Allow the layers to separate, and use the methyl isobutyl ketone (upper) layer for analysis.
Concomitantly determine, at least in triplicate, the absorbances of the Standard solutions
and the Test solution
at 283.3 nm with a suitable atomic absorption spectrophotometer (see Spectrophotometry and Light-Scattering 851
) equipped with a lead hollow-cathode lamp as the radiation source and an airacetylene flame. Record the average steady readings for each of the Standard solutions
and the Test solution.
Plot the absorbances of the Standard solutions
and the Test solution
versus the amount of lead added. Extrapolate the line joining the points on the graph until it meets the concentration axis. The distance between this point and the intersection of the axes represents the concentration of lead in the Test solution:
not more than 0.5 µg per g is found.