Identification—
Solution
: 900 mg per mL. Transfer about 180 mg of it, accurately weighed, to a 200-mL volumetric flask, add 1 mL of lactic acid, dilute with water to volume, and mix. Absorptivities, at about 257 nm, calculated on the dried basis, do not differ by more than 3.0%.
C:
Dissolve about 20 mg in 5 mL of phosphoric acid: a green color is produced.
D:
Dissolve 200 mg in 80 mL of water with the aid of 0.5 mL of 3 N hydrochloric acid, warming, if necessary, to effect solution, and then cool. To 5 mL of the solution add 1 drop of hydrochloric acid and several drops of
mercuric chloride TS: a white precipitate is formed. To a second 5-mL portion of the solution add bromine TS dropwise: a yellow precipitate forms which redissolves on shaking, and finally, upon the addition of more bromine TS, a permanent precipitate is formed.
Assay—
Dissolve about 500 mg of Biperiden, accurately weighed, in 20 mL of benzene, add 2 drops of
crystal violet TS, and titrate with 0.1 N perchloric acid VS to a blue endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 31.15 mg of C
21H
29NO.
;)
-bicyclo[2.2.1]hept-5-en-2-yl-
731
—
Dry it at 105
for 3 hours: it loses not more than 1.0% of its weight.