Limit of (E)-thiothixene
[NOTEPrepare all solutions in low-actinic glassware.
]
Mobile phase
Transfer 6.9 g of monobasic sodium phosphate to a 1-liter volumetric flask, dissolve in and dilute with deionized water to volume, and mix. Filter through a suitable membrane filter. Mix 4 volumes of this solution with 6 volumes of methanol. The concentration of methanol may be adjusted to meet the system suitability requirements.
Standard preparations
A
Using accurately weighed quantities of USP (
E)-Thiothixene RS and
USP Thiothixene RS, prepare a solution in methanol containing, in each mL, 0.4 mg and 1.2 mg, respectively.
B
Transfer 5.0 mL of Standard preparation A to a 100-mL volumetric flask, dilute with methanol to volume, and mix.
C
Transfer about 200 mg of thiothixene, accurately weighed, to a 100-mL volumetric flask. Transfer 5.0 mL of Standard preparation A to the same flask, dissolve in and dilute with methanol to volume, and mix.
Test preparation
Transfer about 200 mg of Thiothixene, accurately weighed, to a 100-mL volumetric flask. Dissolve in methanol, dilute with methanol to volume, and mix.
Procedure
Concomitantly introduce equal volumes (about 20 µL) of
Standard preparation C and
Test preparation into a high-pressure liquid chromatograph operated at room temperature and equipped with a suitable microsyringe or sampling valve, a column containing packing L9 (typically 25 cm × 4.6 mm), an UV detector capable of monitoring absorption at 254 nm, and a suitable recorder. The
Mobile phase is maintained at a flow rate of about 1 to 1.5 mL per minute. In a suitable chromatographic system, three replicate injections of
Standard preparation B show a resolution factor of not less than 2.2 between the thiothixene and (
E)-thiothixene peaks, their retention times being 13 and 15 minutes, and between 16 and 18 minutes, respectively. Calculate the quantity, in mg, of (
E)-thiothixene in the portion of Thiothixene taken by the formula:
5
CHU / (
HC HU),
in which
C is the concentration of USP (
E)-Thiothixene RS, in mg per mL, in
Standard preparation A; and
HC and
HU are the peak responses of the (
E)-thiothixene peaks corrected for the tailing of the main peak, obtained from
Standard preparation C and the
Test preparation, respectively: the limit of (
E)-thiothixene is 1.0%.
Assay
[NOTEPerform the dilution operations in low-actinic glassware.
]
Mobile phase
Mix 0.5 mL of ethanolamine with 3780 mL of methanol, mix 1400 mL of this solution with 200 mL of water, filter, and degas. Make adjustments if necessary (see
System Suitability under
Chromatography 621).
Standard preparation
Using an accurately weighed quantity of
USP Thiothixene RS, prepare a solution in methanol having a known concentration of about 0.02 mg per mL.
Assay preparation
Transfer about 100 mg of Thiothixene, accurately weighed, to a 100-mL volumetric flask, dissolve in and dilute with methanol to volume, and mix. Pipet 2 mL of the resulting solution into a 100-mL volumetric flask, dilute with methanol to volume, and mix.
Chromatographic system
(see
Chromatography 621)The liquid chromatograph is equipped with a 254-nm detector and a 3.9-mm × 30-cm column that contains packing L3. The flow rate is about 0.5 mL per minute. Chromatograph the
Standard preparation, and record the peak responses as directed for
Procedure: the column efficiency determined from the analyte peak is not less than 2000 theoretical plates, and the relative standard deviation for replicate injections is not more than 1.5%.
Procedure
Separately inject equal volumes (about 20 µL) of the
Standard preparation and the
Assay preparation into the chromatograph, record the chromatograms, and measure the responses for the major peaks. Calculate the quantity, in mg, of C
23H
29N
3O
2S
2 in the portion of Thiothixene taken by the formula:
5000C(rU / rS),
in which
C is the concentration, in mg per mL, of
USP Thiothixene RS in the
Standard preparation, and
rU and
rS are the peak responses obtained from the
Assay preparation and the
Standard preparation, respectively.