Packaging and storage—
Preserve in tight, light-resistant containers.
Identification—
B:
Mix a solution (1 in 10) with an equal volume of
ferrous sulfate TS, and superimpose the mixture upon 5 mL of sulfuric acid contained in a test tube: the zone of contact becomes brown.
Melting range 
741
:
between 171
and 176
, with decomposition, but the range between beginning and end of melting does not exceed 3
.
Chloride—
To 5 mL of a solution (1 in 50), acidified with nitric acid, add a few drops of
silver nitrate TS: no opalescence is produced immediately.
Other alkaloids—
Dissolve 200 mg in 20 mL of water, and divide the solution into two portions. To one portion add a few drops of 6 N ammonium hydroxide and to the other add a few drops of
potassium dichromate TS: no turbidity is produced in either solution.
Assay—
Dissolve about 600 mg of Pilocarpine Nitrate, accurately weighed, in 30 mL of glacial acetic acid, warming slightly to effect solution. Cool to room temperature, and titrate with 0.1 N perchloric acid VS, determining the endpoint potentiometrically. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 27.13 mg of C11H16N2O2·NO3.
