Packaging and storage
Preserve in tight, light-resistant containers.
Identification
Solution:
5 µg per mL.
Medium:
alcohol.
Absorptivities at 312 nm do not differ by more than 4.0%.
Chromatographic purity
Chromatographic system
(see
Chromatography 621)The gas chromatograph is equipped with a flame-ionization detector and a 3-mm × 1.8-m stainless steel column packed with 10 percent liquid phase G9 on support S1A. The column temperature is programmed at a rate of 10
per minute from 150
to 250
, then maintained at 250
for 10 minutes, and helium is used as the carrier gas.
Procedure
Chromatograph 2 µL of a 1 in 100 solution of Padimate O in chloroform: the response due to padimate O is not less than 98.0% of the sum of the responses on the chromatogram, exclusive of the chloroform peak.
Assay
Dissolve about 500 mg of Padimate O, accurately weighed, in 75 mL of acetic anhydride, and titrate with 0.1 N perchloric acid VS, determining the endpoint potentiometrically. Each mL of 0.1 N perchloric acid is equivalent to 27.74 mg of C17H27NO2.