Packaging and storage—
Preserve in tight, light-resistant containers.
Identification—
Solution:
20 µg per mL.
Medium:
methanol.
Absorptivities at 280 nm, calculated on the dried basis, do not differ by more than 3.0%.
C:
A solution (1 in 100) responds to the tests for
Chloride 
191
.
pH 791:
between 5.0 and 6.6, in a 1 in 100 solution in carbon dioxide-free water, and the solution is clear and colorless.
Ordinary impurities 466—
Test solution:
methanol.
Standard solution:
methanol.
Eluant:
a mixture of methanol, glacial acetic acid, and water (8:1:1).
Visualization:
2.
Assay—
Dissolve about 300 mg of Naphazoline Hydrochloride, accurately weighed, in 50 mL of glacial acetic acid, and add 10 mL of
mercuric acetate TS and 1 drop of
crystal violet TS. Titrate (see
Titrimetry 541) with 0.1 N perchloric acid VS to a blue-green endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 24.67 mg of C
14H
14N
2·HCl.
Auxiliary Information—
Staff Liaison :
Feiwen Mao, M.S., Senior Scientific Associate
Expert Committee : (MDOOD05) Monograph Development-Ophthalmics Oncologics and Dermatologicals
USP29–NF24 Page 1480
Pharmacopeial Forum : Volume No. 31(4) Page 1093
Phone Number : 1-301-816-8320
;)
for 2 hours: it loses not more than 0.5% of its weight.