B:
To 0.5 mL of a solution (1 in 2000) add 1 mL of
Folin-Ciocalteu phenol TS, then add 5 mL of sodium carbonate solution (1 in 10), mix, and allow to stand for 5 minutes: an intense blue color appears
(presence of a phenol).
C:
To 4 mL of a solution (1 in 2000) add 5 mL of pH 9.6 alkaline borate buffer (see
Buffer Solutions in the section
Reagents, Indicators, and Solutions), then add about 5 mg of
![](images/chars/beta.gif)
-naphthoquinone-4-sodium sulfonate, mix until dissolved, and allow to stand for 5 minutes. Add 0.2 mL of benzalkonium chloride solution (1 in 100), mix, add 5 mL of toluene, and shake: the toluene layer turns purple immediately
(distinction from phenylephrine).