Assay
Assay preparation
Using the dosing pump, deliver a number of doses of Oral Suspension, equivalent to about 250 mg of amoxicillin, to a separator containing 100 mL of hexanes, and shake vigorously. Add 140 mL of water, and shake for 5 minutes. Allow the layers to separate, and drain the lower, aqueous layer into a 250-mL volumetric flask. Repeat the extraction with two 50-mL portions of water. Combine the aqueous extracts in the volumetric flask, dilute with water to volume, and mix.
Procedure
Proceed with Oral Suspension as directed for
Procedure under
Iodometric AssayAntibiotics 425, using
USP Amoxicillin RS. Calculate the quantity, in mg, of amoxicillin (C
16H
19N
3O
5S) in each dose of Oral Suspension taken by the formula:
(250 /
N)(
F / 2000)(
B I),
in which
N is the number of doses taken, and the other terms are as defined therein.