Use healthy, mature rabbits. House the rabbits individually in an area of uniform temperature between 20
and 23
and free from disturbances likely to excite them. The temperature varies not more than ±3
from the selected temperature. Before using a rabbit for the first time in a pyrogen test, condition it not more than seven days before use by a sham test that includes all of the steps as directed for
Procedure except injection. Do not use a rabbit for pyrogen testing more frequently than once every 48 hours, nor prior to 2 weeks following a maximum rise of its temperature of 0.6
or more while being subjected to the pyrogen test, or following its having been given a test specimen that was adjudged pyrogenic.